13916964679
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production. In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade. In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86. Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway. In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner. .
Toll-like receptor 4 (Toll4) (CD antigen CD284) Tlr4
Rat,Mouse
IHC,IF,ICC
IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
97kD
Tlr4
Q9QUK6,Q9QX05
Mouse
Monoclonal
Mouse IgG
Product Information
| Description | TLR4 mouse monoclonal |
| Protein full name | Toll-like receptor 4 |
Synonyms | TLR4, ARMD10, CD284, TLR-4, TOLL,toll like receptor 4 |
| Immunogen | Recombinant protein corresponding toMouse TLR4 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cell membrane |
| Uniprot ID |
Applications
| Applications | IHC/IF | ||
| Species | Mouse,Rat | ||
| Dilution | 1:500-1:1000 | ||
| Positive tissue | spleen |
Background
TLR4is a transmembrane protein, member of thetoll-like receptorfamily, which belongs to thepattern recognition receptor(PRR) family. Its activation leads to an intracellular signaling pathwayNF-κBand inflammatory cytokine production which is responsible for activating the innate immune system.It is most well known for recognizinglipopolysaccharide(LPS), a component present in manyGram-negative bacteria(e.g.Neisseriaspp.) and selectGram-positive bacteria. Its ligands also include several viral proteins, polysaccharide, and a variety of endogenous proteins such as low-density lipoprotein, beta-defensins, and heat shock protein.
Images
| Immunohistochemistry analysis of paraffin-embedded mouse spleen using TLR4(T62186M) at dilution of 1:1000 |
| Immunohistochemistry analysis of paraffin-embedded rat spleen using TLR4(T62186M) at dilution of 1:1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
AB_2920650
艾比玛特医药科技（上海）有限公司
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微信客服
邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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