13916964679
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
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Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex . Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions . Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases . Ubiquitinates, in an oxygen-responsive manner, ADRB2 . Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR . .
von Hippel-Lindau disease tumor suppressor (Protein G7) (pVHL) VHL
Human
IHC,IF,ICC
IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
25kD
VHL
P40337
Mouse
Monoclonal
Mouse IgG
negative regulation of signal transduction
Product Information
| Gene Name | VHL |
| Protein Name | Von Hippel-Lindau disease tumor suppressor (Protein G7) (pVHL) |
| Human Gene Id | 7428 |
| Human Swiss Prot No | P40337 |
| Immunogen | Synthesized peptide derived from human pVHL |
| Specificity | This antibody detects endogenous levels of human pVHL |
| Source | Mouse/IgG1, Kappa |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Applications
| Applications | Species | Dilution | |
| IHC、IF | Human | 1:200-1:500 |
Background
von Hippel-Lindau tumor suppressor(VHL) Homo sapiens Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
Images
 | Immunohistochemical analysis of paraffin-embedded Kindey. 1, Antibody was diluted at 1:200(4deg;,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. |
 | Immunohistochemical analysis of paraffin-embedded Kindey. 1, Antibody was diluted at 1:200(4deg;,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
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