Recombinant protein of human leucine aminopeptidase 3 (LAP3)

P28838

AMPL,LAP3 LAPEP PEPS,Cytosol aminopeptidase, EC 3.4.11.1 (Cysteinylglycine-S-conjugate dipeptidase, EC 3.4.13.23) (Leucine aminopeptidase 3, LAP-3) (Leucyl aminopeptidase) (Peptidase S) (Proline aminopeptidase, EC 3.4.11.5) (Prolyl aminopeptidase)

56 kDa

HEK293T

Human

> 80% as determined by SDS-PAGE and Coomassie blue staining

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

C-Myc/DDK

>Protein sequenceRed=Cloning site Green=Tags(s)MFLLPLPAAGRVVVRRLAVRRFGSRSLSTADMTKGLVLGIYSKEKEDDVPQFTSAGENFDKLLAGKLRETLNISGPPLKAGKTRTFYGLHQDFPSVVLVGLGKKAAGIDEQENWHEGKENIRAAVAAGCRQIQDLELSSVEVDPCGDAQAAAEGAVLGLYEYDDLKQKKKMAVSAKLYGSGDQEAWQKGVLFASGQNLARQLMETPANEMTPTRFAEIIEKNLKSASSKTEVHIRPKSWIEEQAMGSFLSVAKGSDEPPVFLEIHYKGSPNANEPPLVFVGKGITFDSGGISIKASANMDLMRADMGGAATICSAIVSAAKLNLPINIIGLAPLCENMPSGKANKPGDVVRAKNGKTIQVDNTDAEGRLILADALCYAHTFNPKVILNAATLTGAMDVALGSGATGVFTNSSWLWNKLFEASIETGDRVWRMPLFEHYTRQVVDCQLADVNNIGKYRSAGACTAAAFLKEFVTHPKWAHLDIAGVMTNKDEVPYLRKGMTGRPTRTLIEFLLRFSQDNATRTRPLEQKLISEEDLAANDILDYKDDDDKV

5 工作日

Arginine and proline metabolism, Glutathione metabolism, Metabolic pathways

51056

Store at -80°C.

LAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, RG309052) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 μl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours.

>50 ug/mL as determined by microplate BCA method