soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM, sCD40 Ligand

Measure by its ability to induce IL-8 secretion in human PBMCs.
The ED50 for this effect is <5 ng/mL.

CD40, belonging to the TNF receptor family, is a cell surface protein generally expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and on T cells at low levels. The membrane-anchored CD40 Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. CD40 signaling plays a significant role immunoglobulin (Ig) class switching, and is critical for the proliferation and differentiation of B cells. A disease "immunodeficiency with hyper-IgM", characterized by failure to produce IgG, IgA and IgE due to failure expression of CD40L. The soluble form of CD40L is produced in vivo by an intracellular proteolytic processing of the entire TNF homologous region (18 kDa protein) of CD40L.

P29965

959

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL with polyhistidine tag at the C-terminus

His-tag at the C-terminus

Human

Human

Escherichia coli

E. coli

>95% as determined by SDS-PAGE.
Ni-NTA chromatography.

<0.1 EU per 1 μg of the protein by the LAL method.

16.99 kDa

The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Blue Ice

Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Cytokines

Cell culture, Elisa