Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
One distinctive HIV antigen is a viral protein called p24, a structural protein that makes up most of the HIV viral core, or 'capsid'. There are approximately 2000 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. The onset of symptoms of AIDS correlates with a reduction in the number of CD4+ T-cells and increased levels of virus and p24 in the blood. It is a component of the gag polyprotein. High levels of p24 are present in the blood serum of newly infected individuals during the short period between infection and seroconversion, making p24 antigen assays useful in diagnosing primary HIV infection. Antibodies to p24 are produced during seroconversion, rendering p24 antigen undetectable after seroconversion in most cases. Therefore, p24 antigen assays are not reliable for diagnosing HIV infection after its very earliest stages. Fourth-generation HIV immunoassays detect viral p24 protein in the blood (as well as patient antibodies against the virus). Previous generation tests relied on detecting patient antibodies alone; it takes about 3–4 weeks for the earliest antibodies to be detected. The p24 protein can be detected in patient blood as early as 2 weeks after HIV infection, further reducing the window period necessary to accurately detect the HIV status of the patient.
Gag-Pol polyprotein (Pr160Gag-Pol) [Cleaved into: Matrix protein p17 (MA); Capsid protein p24 (CA); Spacer peptide 1 (SP1) (p2); Nucleocapsid protein p7 (NC); Transframe peptide (TF); p6-pol (p6*); Protease (EC 3.4.23.16) (PR) (Retropepsin); Reverse transcriptase/ribonuclease H (EC 2.7.7.49) (EC 2.7.7.7) (EC 3.1.26.13) (Exoribonuclease H) (EC 3.1.13.2) (p66 RT); p51 RT; p15; Integrase (IN) (EC 2.7.7.-) (EC 3.1.-.-)] gag-pol
Hiv1
ELISA(cap)
ELISA 1:5000-1:20000
Predicted band size: 24 kDa
gag-pol
p12497
Mouse
Monoclonal
Mouse IgG1
DNA integration #DNA recombination #Activation of host caspases by virus #Modulation of host cell apoptosis by virus #Host gene expression shutoff by virus #Virus entry into host cell #Viral genome integration #Viral penetration into host nucleus #DNA recombination #proteolysis,
Recombanint HIV1 p24 protein.
Protein A affinity purified.
PBS (pH7.4)
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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