IHC-P analysis of brain tissue by NEFH antibody.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with NEFH antibody at 1 μg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.

Result: Neuropil are positively stained.

Microtiter wells were coated with ME9240703 at 3 μg/mL as the capture antibody. NEFH was used as the antigen. Peroxidase conjugated mouse anti-human NEFH monoclonal antibody (ME012919) was used as the detection antibody.

Result: ME9240703 and ME012919 can be used as a matched antibody pair to detect and quantify the concentration of NEFH.