PH10245

货号 规格 价格(¥)
PH10245S 50ul
PH10245M 100ul

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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   蛋白质免疫共沉淀

   蛋白质印迹

   免疫荧光

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   膜再生

   植物BPP法变性蛋白提取

   组织制备成单细胞悬液

经典一抗-cas9 Antibody

Datasheet

Background

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (Probable). Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer yielding blunt ends; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes a 3'-G-rich protospacer adjacent motif (PAM, TGGTG in this organism) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming. .

Alternative Name

CRISPR-associated endonuclease Cas9 (EC 3.1.-.-) (St-Cas9) cas9 csn1

Reactivity

Streptococcus thermophilus

Predicted Reactivity

Application

WB,ELISA

Application Image

Recommended Dilution

WB 1:1000-1:2000
ELISA 1:5000-1:20000

Mol Weight

164kD

Gene Symbol

cas9 csn1

Uniprot

g3ecr1

Uniprot link

Immunogen

Recombinant Streptococcus thermophilus cas9 protein (785-983aa )

Immunogen Range

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Specificity

Subcellular location

Research Area

Others

Positive control

Conjugation

Modification

Purification

Protein G

Formulation

Liquid

Concentration

1mg/ml

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

产品介绍

Clone

Target

波长

Storage

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.

注意事项

Companion Products

Important

Comments

RRID

客户评价

艾比玛特医药科技(上海)有限公司
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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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