E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation . Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs . This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy . Upon enteroviral infection, promotes ''Lys-63''-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade . Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs . Plays also a role in limitation of inflammation through different mechanisms. First, promotes ''Lys-48''-mediated ubiquitination of VCAM1 leading to its degradation and limitation of LPS-induced lung inflammation . In addition, negatively regulates inflammasome activation by promoting ''lys48''-linked ubiquitination of NLRP3 which is critical for the inhibition of NLRP3 inflammasome activation in resting macrophages . .

E3 ubiquitin-protein ligase TRIM65 (EC 2.3.2.27) (Tripartite motif-containing protein 65),TRIM65

q6pj69

Mouse

Monoclonal

IgG1

DAERLKREAQLRASLEVTQQQATQAEGQLLELRKQSSQIQNSACILASWVSGKFSSLLQALEIQHTTALRSIEVAKTQALAQARDEEQRLRVHLEAVARHGCRIRELLEQVDEQTFLQESQLLQPPGPLGPLTPLQWDEDQQLGDLKQLLSRLCGLLLEEGSHPGAPAKPVDLAPVEAPG

Human

WB

WB 1:1000

57kD

Supplied as solution in phosphate buffered saline containing 0.09% sodium azide

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.