TU22037

货号 规格 价格(¥)
TU22037S 50ul
TU22037M 100ul

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

ABMART实验方案下载

   蛋白质免疫共沉淀

   蛋白质印迹

   免疫荧光

   免疫组化

   膜再生

   植物BPP法变性蛋白提取

   组织制备成单细胞悬液

经典一抗-Pan-Lactyl-lysine Antibody

Datasheet

Background

Post-translational modifications (PTMs) represent a crucial means of regulating diverse biological processes and cellular physiology by influencing protein structure and function. Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300. This modification was high in gene promoter regions that lack acetylation and associated with activation of genes expression. As a large number of studies have demonstrated that lysine acylation possessed a diverse range of substrate proteins, however, no systematic analysis has been reported for lysine lactylation.

Alternative Name

Lactyllysine antibody

Reactivity

All

Application

WB,IHC,IF,ICC,Dot Blot

Recommended Dilution

WB 1:2000
IHC 1:500-1:1000
IF 1:100-1:400
ICC 1:1000
Dot Blot 1:2000

Application Image

Mol Weight

Gene Symbol

Uniprot

n/a

Uniprot link

Immunogen

Immunogen Range

Source

Rabbit

Clonality

Recombinant Rabbit monoclonal Antibody

Isotype

IgG

Specificity

Subcellular location

Nucleus.

Research Area

Positive control

HeLa treated with 100mM Lactate sodium for 24 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue, HeLa cells treated with 100mM Lactate sodium for 24 hours.

Conjugation

Modification

Purification

Protein A affinity purified.

Formulation

Liquid

Concentration

1 mg/mL.

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

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技术支持:15618194176(微信同号)

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北方及西南经销商负责:

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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