p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexes, and thus functions as a regulator of cell cycle progression at G1 and S phase. The binding of p21 to CDK complexes occurs through p21's N-terminal domain, which is homologous to the other CIP/KIP CDK inhibitors p27 and p57. Specifically it contains a Cy1 motif in the N-terminal half, and weaker Cy2 motif in the C-terminal domain that allow it to bind CDK in a region that blocks its ability to complex with cyclins and thus prevent CDK activation. Experiments looking at CDK2 activity within single cells have also shown p21 to be responsible for a bifurcation in CDK2 activity following mitosis, cells with high p21 enter a G0/quiescent state, whilst those with low p21 continue to proliferate. Follow up work, found evidence that this bistability is underpinned by double negative feedback between p21 and CDK2, where CDK2 inhibits p21 activity via ubiquitin ligase activity.

CAP20 antibody
CDK-interacting protein 1 antibody
CDKI antibody
CDKN1 antibody
Cdkn1a antibody
CDN1A_HUMAN antibody
CIP1 antibody
Cyclin Dependent Kinase Inhibitor 1A antibody
Cyclin-dependent kinase inhibitor 1 antibody
Cyclin-dependent kinase inhibitor 1A (P21) antibody
Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody
DNA Synthesis Inhibitor antibody
MDA-6 antibody
MDA6 antibody
Melanoma differentiation-associated protein 6 antibody
Melanoma differentiation-associated protein antibody
p21 antibody
P21 protein antibody
p21CIP1 antibody
p21Cip1/Waf1 antibody
p21WAF antibody
PIC1 antibody
SDI1 antibody
SLC12A9 antibody
WAF1 antibody
Wild type p53 activated fragment 1 (WAF1) antibody
Wild type p53 activated fragment 1 antibody
Wildtype p53-activated fragment 1 antibody

Mouse

WB,IHC,IF,ICC,FC

WB 1:1000
IHC 1:200
IF 1:100-1:400
ICC 1:100
FC 1:1000

Predicted band size: 18 kDa

Cdkn1a, Cip1, Waf1.

p39689>swissprot: p39689 mouse

Rabbit

Recombinant Rabbit monoclonal Antibody

IgG

Cytoplasm, Nucleus.

NIH/3T3 cell lysate, RAW264.7 cell lysate, RAW264.7, mouse testis tissue.

Protein A affinity purified.

Liquid

1 mg/mL.

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.