DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that in humans is encoded by the gene designated as PRKDC or XRCC7. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. The DNA-Pkcs protein is a serine/threonine protein kinase consisting of a single polypeptide chain of 4,128 amino acids. DNA-PKcs is the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase called DNA-PK. The second component is the autoimmune antigen Ku. On its own, DNA-PKcs is inactive and relies on Ku to direct it to DNA ends and trigger its kinase activity. DNA-PKcs is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V(D)J recombination, a process that utilizes NHEJ to promote immune system diversity. Many proteins have been identified as substrates for the kinase activity of DNA-PK. Autophosphorylation of DNA-PKcs appears to play a key role in NHEJ and is thought to induce a conformational change that allows end processing enzymes to access the ends of the double-strand break. DNA-PK also cooperates with ATR and ATM to phosphorylate proteins involved in the DNA damage checkpoint.

DNA dependent protein kinase catalytic subunit antibody
DNA PK catalytic subunit antibody
DNA-dependent protein kinase catalytic subunit antibody
DNA-PK catalytic subunit antibody
DNA-PKcs antibody
DNAPK antibody
DNAPK catalytic subunit antibody
DNPK 1 antibody
DNPK1 antibody
Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody
HYRC 1 antibody
HYRC antibody
HYRC1 antibody
IMD26 antibody
p350 antibody
p460 antibody
PKRDC antibody
PRKDC antibody
PRKDC_HUMAN antibody
Protein Kinase DNA Activated Catalytic Polypeptide antibody
XRCC 7 antibody
XRCC7 antibody

Human

WB,IF,ICC

WB 1:1000
IF 1:100-1:400
ICC 1:100

Predicted band size: 469 kDa

PRKDC, HYRC, HYRC1

p78527>swissprot: p78527 human

Rabbit

Recombinant Rabbit monoclonal Antibody

IgG

Nucleus, nucleolus.

Cell Biology->Apoptosis;Cell Biology->Apoptosis->Nucleus->Other;Cancer->Cell Death;Cancer->Cell Death->Apoptosis->Nucleus->Other;Signal Transduction->Protein Phosphorylation;Signal Transduction->Protein Phosphorylation->Ser / Thr Kinases->Other Kinases;Epigenetics and Nuclear Signaling->DNA / RNA;Signal Transduction->Caspase Cascade

HT-29 treated with UV for 3 hours cell lysate, HT-29 cells treated with UV for 3 hours.

Protein A affinity purified.

Liquid

1 mg/mL.

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.