RAC(Rho family)-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) protein domains. It is commonly referred to as PKB, or by both names as "Akt/PKB". The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Phospho-Akt (Ser473) [PSH04-44] Recombinant Rabbit mAb detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.

AKT1 antibody
AKT1_HUMAN antibody
MGC99656 antibody
PKB antibody
PKB-ALPHA antibody
PRKBA antibody
Protein Kinase B Alpha antibody
Protein kinase B antibody
Proto-oncogene c-Akt antibody
RAC Alpha antibody
RAC antibody
RAC-alpha serine/threonine-protein kinase antibody
RAC-PK-alpha antibody
akt s473 antibody

Human,Mouse,Rat

WB,IHC,FC

WB 1:2000-1:5000
IHC 1:200
FC 1:10000

Predicted band size: 56 kDa

RAC-alpha serine/threonine-protein kinase
RAC-beta serine/threonine-protein kinase
RAC-gamma serine/threonine-protein kinase
Protein kinase B (PKB)
Protein kinase Akt-2
Protein kinase Akt-3
Protein kinase B alpha (PKB alpha)
Protein kinase B beta (PKB beta)
Protein kinase B gamma (PKB gamma)
Proto-oncogene c-Akt
RAC-PK-alpha
RAC-PK-beta
RAC-PK-gamma
AKT1
AKT2
AKT3
PKB
PKBG
RAC
STK-2
akt s473

p31749>swissprot: p31749 human;swissprot: p31751 human;swissprot: q9y243 human;swissprot: p31750 mouse;swissprot: q60823 mouse;swissprot: q9wua6 mouse;swissprot: p47196 rat;swissprot: p47197 rat;swissprot: q63484 rat

Rabbit

Recombinant Rabbit monoclonal Antibody

IgG

Cytoplasm, Nucleus, Cell membrane.

HEK-293 cell lysate, C6 cell lysate, C6 treated with 100ng/mL PDGF for 5 minutes cell lysate, MCF7 cell lysate, MCF7 treated with 100nM Calyculin A for 30 minutes cell lysate, SH-SY5Y cell lysate, SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate.

Protein A affinity purified.

Liquid

1 mg/mL.

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.