TU22148

货号 规格 价格(¥)
TU22148S 50ul
TU22148M 100ul

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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经典一抗-Phospho-Rad17 (S656) Antibody

Datasheet

Background

Cell cycle checkpoint protein RAD17 is a protein that in humans is encoded by the RAD17 gene. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Phospho-Rad17 (S656) Antibody [JE63-30] recognizes endogenous levels of Rad17 protein only when phosphorylated at Ser656.

Alternative Name

CCYC antibody
Cell cycle checkpoint protein antibody
Cell cycle checkpoint protein RAD17 antibody
FLJ41520 antibody
HRAD 17 antibody
hRad17 antibody
R24L antibody
Rad 17 antibody
Rad 24 antibody
RAD1 (S. pombe) homolog antibody
RAD1 homolog antibody
RAD17 antibody
RAD17 homolog (S. pombe) antibody
RAD17 homolog antibody
Rad17 like protein antibody
RAD17, S. pombe, homolog of antibody
RAD17_HUMAN antibody
RAD17Sp antibody
Rad24 antibody
Rad24, mouse, homolog of antibody
Rad24, S. cerevisiae, homolog of antibody
RF C activator 1 homolog antibody
RF C/activator 1 homolog antibody
RF-C/activator 1 homolog antibody

Reactivity

Human,Mouse

Application

WB,IF,ICC,FC

Recommended Dilution

WB 1:1000
IF 1:100-1:400
ICC 1:500
FC 1:1000

Application Image

Mol Weight

Predicted band size: 77 kDa

Gene Symbol

RAD17, R24L

Uniprot

o75943>swissprot: o75943 human;swissprot: q6nxw6 mouse

Uniprot link

Immunogen

Immunogen Range

Source

Rabbit

Clonality

Recombinant Rabbit monoclonal Antibody

Isotype

IgG

Specificity

Subcellular location

Nucleus.

Research Area

Epigenetics and Nuclear Signaling->DNA / RNA

Positive control

HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, HeLa cells treated with 100ng/mL Calyculin A for 30 minutes.

Conjugation

Modification

Purification

Protein A affinity purified.

Formulation

Liquid

Concentration

1 mg/mL.

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

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技术支持:15618194176(微信同号)

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北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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