The cellular level of β-catenin is mostly controlled by its ubiquitination and proteosomal degradation. The E3 ubiquitin ligase TrCP1 (also known as β-TrCP) can recognize β-catenin as its substrate through a short linear motif on the disordered N-terminus. However, this motif (Asp-Ser-Gly-Ile-His-Ser) of β-catenin needs to be phosphorylated on the two serines in order to be capable to bind β-TrCP. Phosphorylation of the motif is performed by Glycogen Synthase Kinase 3 alpha and beta (GSK3α and GSK3β). GSK3s are constitutively active enzymes implicated in several important regulatory processes. There is one requirement, though: substrates of GSK3 need to be pre-phosphorylated four amino acids downstream (C-terminally) of the actual target site. Thus it also requires a "priming kinase" for its activities. In the case of β-catenin, the most important priming kinase is Casein Kinase I (CKI). Once a serine-threonine rich substrate has been "primed", GSK3 can "walk" across it from C-terminal to N-terminal direction, phosphorylating every 4th serine or threonine residues in a row. This process will result in dual phosphorylation of the aforementioned β-TrCP recognition motif as well.

b-catenin antibody
Beta catenin antibody
Beta-catenin antibody
Cadherin associated protein antibody
Catenin (cadherin associated protein), beta 1, 88kDa antibody
Catenin beta 1 antibody
Catenin beta-1 antibody
CATNB antibody
CHBCAT antibody
CTNB1_HUMAN antibody
CTNNB antibody
CTNNB1 antibody
DKFZp686D02253 antibody
FLJ25606 antibody
FLJ37923 antibody
OTTHUMP00000162082 antibody
OTTHUMP00000165222 antibody
OTTHUMP00000165223 antibody
OTTHUMP00000209288 antibody
OTTHUMP00000209289 antibody

Human,Mouse,Rat

WB,IHC

WB 1:1000
IHC 1:200

Predicted band size: 85 kDa

CTNNB1, CTNNB

p35222>swissprot: p35222 human;swissprot: q02248 mouse;swissprot: q9wu82 rat

Rabbit

Recombinant Rabbit monoclonal Antibody

IgG

Cytoplasm, Nucleus, Cell membrane.

Cancer->Invasion/microenvironment->ECM->Cell adhesion->Cadherins;Signal Transduction->Cytoskeleton / ECM->Cytoskeleton->Microfilaments->Actin etc->Catenins;Signal Transduction->Cytoskeleton / ECM->Cell Adhesion->Cadherins;Cardiovascular->Atherosclerosis->Vascular Inflammation->Leukocyte recruitment->Other;Epigenetics and Nuclear Signaling->ChIP assays->ChIP kits;Epigenetics and Nuclear Signaling->ChIP assays->ChIP antibodies;Epigenetics and Nuclear Signaling->Ubiquitin & Ubiquitin Like Modifiers->Deubiquitination

HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Protein A affinity purified.

Liquid

1 mg/mL.

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.