Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. It is the archaeal and eukaryotic counterpart of bacterial EF-G. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the ribosome. This protein is completely inactivated by EF-2 kinase phosphorylation. aEF2/eEF2 found in most archaea and eukaryotes, including humans, contains a post translationally modified histidine diphthamide. It is the target of diphtheria toxin (from Corynebacterium diphtheriae), and exotoxin A (from Pseudomonas aeruginosa). The inactivation of EF-2 by toxins inhibits protein production in the host, causing symptoms due to loss of function in affected cells.

EEF 2 antibody
Eef2 antibody
EF-2 antibody
EF2 antibody
EF2_HUMAN antibody
Elongation factor 2 antibody
Eukaryotic translation elongation factor 2 antibody
Polypeptidyl tRNA translocase antibody
SCA26 antibody

Human,Mouse,Rat

WB,IHC,IF,ICC

WB 1:2000
IHC 1:500
IF 1:100-1:400
ICC 1:100

Predicted band size: 95 kDa

EEF2, EF2

p13639>swissprot: p13639 human;swissprot: p58252 mouse;swissprot: p05197 rat

Rabbit

Recombinant Rabbit monoclonal Antibody

IgG

Cytoplasm, Nucleus.

Epigenetics and Nuclear Signaling

HeLa starved for 24 hours then add 10μM forskolin for 1 hour cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved for 24 hours then add 10μM forskolin for 1 hour cell lysate, PC-12 treated with 20μM forskolin for 1 hour then add 100nM Calyculin A for 30 minutes cell lysate, HepG2 cell lysate, rat liver tissue, HeLa cells starved for 24 hours then add 10μM forskolin for 1 hour, PC-12 cells treated with 20μM forskolin for 1 hour then add 100nM Calyculin A for 30 minutes.

Protein A affinity purified.

Liquid

1 mg/mL.

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.