S100P binding protein Rabbit pAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC6577

货号规格价格(￥)

PC6577S 50ul

PC6577M 100ul

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-S100P binding protein Rabbit pAb

Datasheet

Background

S100PBP was originally cloned from a pancreatic epithelioid carcinoma library and encodes a predicted 408 amino acid protein. RT-PCR detected S100PBP expression in brain, breast, spleen, and lung, but not in pancreas and liver. GFP-tagged S100PBP localized to nuclei of transfected HeLa cells.

Alternative Names

S100P-binding protein; S100P-binding protein Riken;y S100PBP; S100PBPR; S1PBP_HUMAN.

Reactivity

Human,Mouse,Rat

Application

WB,FC

Recommended Dilution

WB 1:500-2000
FC 1ug/Test

Application Image

Mol Weight

46kD

Gene ID

64766

UniProt

Q96BU1

Uniprot link

https://www.uniprot.org/uniprotkb/Q96BU1

Subcellular location

Nucleus. Note=Colocalizes with S100P in the nucleus.

Specificity

Expressed in brain, spleen, and lung. Not detected in pancreas or liver. In pancreas, expressed predominantly in islet cells and to a lesser extent in acinar cells, but not expressed in ductal cells. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. Detected in pancreatic ductal adenocarcinoma cells (at protein level). Not detected in non-neoplastic ductal epithelium (at protein level).

Immunogen

KLH conjugated synthetic peptide derived from human S100PBP

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Sample: Spleen (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti- S100P binding protein (PC6577) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD
	Sample: Molt-4 (Human) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti- S100P binding protein (PC6577) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD

	Blank control:K562. Primary Antibody (green line): Rabbit Anti-S100P binding protein antibody (PC6577) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
	Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-S100P binding protein antibody (PC6577) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
	Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-S100P binding protein antibody (PC6577) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488R Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.