This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008].

Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform; dUTP pyrophosphatase; dUTPase; FLJ20622; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; Deoxyuridine triphosphatase; dut; DUT_HUMAN; dUTP nucleotidohydrolase.

Human,Mouse,Rat,Dog,Pig,Cow,Rabbit

IHC,IF,ICC

IHC 1:100-500
IF 1:100-500
ICC 1:100-1:400

20kD

1854

P33316

https://www.uniprot.org/uniprotkb/P33316

Isoform 2: Nucleus. Isoform 3: Mitochondrion.

Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).

KLH conjugated synthetic peptide derived from human dUTPase

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.