Adiponectin receptor 2 Rabbit pAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC0611

货号规格价格(￥)

PC0611S 50ul

PC0611M 100ul

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Adiponectin receptor 2 Rabbit pAb

Datasheet

Background

The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008]

Alternative Names

Adipo R2; Progestin and adipoQ receptor family member II; Adipor2; PAQR2; ADR2_HUMAN.

Reactivity

Human,Mouse,Rat,Chicken,Dog,Pig,Cow

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

44kD

Gene ID

79602

UniProt

Q86V24

Uniprot link

https://www.uniprot.org/uniprotkb/Q86V24

Subcellular location

Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles.

Specificity

Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.

Immunogen

KLH conjugated synthetic peptide derived from human Adiponectin receptor 2

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(PC0611) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(PC0611) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (PC0611) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.

Blank control: HepG2(blue). Primary Antibody:Rabbit Anti-Adiponectin receptor 2 antibody (PC0611,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (PC0611, 0.2μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: Skeletal muscle (Rat) Lysate at 40 ug Lane 5: 293T (Human) Cell Lysate at 30 ug Primary: Anti-Adiponectin receptor 2 (PC0611) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 43 kD