Beclin 1 Mouse mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MC33323

货号规格价格(￥)

MC33323S 50ul

MC33323M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Beclin 1 Mouse mAb

Datasheet

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.

Alternative Names

Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.

Reactivity

Human,Mouse,Rat

Application

IHC,IF,ICC

Recommended Dilution

IHC 1:100-500
IF 1:100-500
ICC 1:100-1:400

Application Image

Mol Weight

50kD

Gene ID

8678

UniProt

Q14457

Uniprot link

https://www.uniprot.org/uniprotkb/Q14457

Subcellular location

Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

Specificity

Ubiquitous.

Immunogen

KLH conjugated synthetic peptide derived from human Beclin 1

Source

Mouse

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein G

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (ascites of MC33323 7B12) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (MC33323-7B12) at 1:400 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Monoclonal Antibody, Unconjugated (MC33323-7B12) at 1:500 overnight at 4°C, followed by a conjugated secondary (A10026) for 20 minutes and DAB staining.