Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (ascites of MC33193 6B10) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (ascites of MC33193 6B10) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:400 overnight at 4°C, followed by a conjugated secondary (A10026) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (MC33193) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Cytochrome C) monoclonal Antibody, Unconjugated (MC33193) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Hcclm3(Human) Cell Lysate at 30 ug Primary: Anti-Cytochrome C (MC33193) at 1/100000 dilution Secondary: Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 14 kD Observed band size: 14 kD

Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Muscle tissue lysates Lane 3: Mouse Cerebrum tissue lysates Lane 4: Rat Muscle tissue lysates Lane 5: Human Jurkat cell lysates Lane 6: Human HL-60 cell lysates Lane 7: Human U251 cell lysates Primary: Anti-Cytochrome C (MC33193) at 1/2000 dilution Secondary: Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa