Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors (1,2). These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types (1,3). The majority of TNF functions are primarily mediated through TNF-R1 (1,4). FAN (for factor associated with neutral sphingomyelinase (N-SMase) activation) is an intermediate protein that interacts with TNF-R1 to initiate TNF signaling events. FAN binds to TNF-R1 at the cytoplasmic NSD (N-SMase activating domain), which results in the initiation of the N-SMase pathway (5). N-SMase has been shown to be involved in TNF-induced Raf-1 activation (6). FAN contains four carboxy-terminal WD-repeat domains which appear to be involved in protein-protein interaction. The FAN WD-repeats may mediate the interaction between FAN and TNF-R1 (5).

Cas/HEF1 associated signal transducer;
CHAT;
FLJ39664;
Novel SH2 containing protein 3;
Novel SH2-containing protein 3;
NSP 3;
PRO34088;
SH2 domain containing 3C;
SH2 domain containing Eph receptor binding protein 1;
SH2 domain containing protein 3C;
SH2 domain-containing protein 3C;
SH2D3_HUMAN;
SH2D3C;
Shep1.

Human,Mouse,Rat,Dog,Cow

WB,IHC,IF,ICC,ELISA

WB 1:500-2000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:100-1:400
ELISA 1:5000-10000

94kD

10044

Q8N5H7

https://www.uniprot.org/uniprotkb/Q8N5H7

Cytoplasm. Membrane. Associated with the membrane when EGF-stimulated. Found at ruffling membranes.

Ubiquitously expressed.

KLH conjugated synthetic peptide derived from human NSP3

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.