Calreticulin Rabbit mAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

TC52436

货号规格价格(￥)

TC52436S 50ul

TC52436M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Calreticulin Rabbit mAb

Datasheet

Background

Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]

Alternative Names

Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:50-200
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

44kD

Gene ID

811

UniProt

P27797

Uniprot link

https://www.uniprot.org/uniprotkb/P27797

Subcellular location

Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.

Specificity

Immunogen

KLH conjugated synthetic peptide derived from human Calreticulin

Source

Rabbit

Clonality

Recombinant

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated(TC52436) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026)and DAB staining.