Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, which lack typical C-terminal amino acid motifs for isoprenylation. Rad and Gem bind calmodulin in a Ca2+-dependent manner via this C-terminal extension, involving residues 278–297 in human Rad. High intracellular Gem levels, which interact with intact microtubules and microfilaments, promote profound changes in cell morphology. Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons. Gem was also observed in developing trigeminal nerve ganglia in 12.5 day mouse embryos, demonstrating that Gem expression is a property of normal ganglionic development. The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface. The human Gem gene maps to chromosome 8q22.1.

GTP binding mitogen induced T cell protein; GTP binding protein expressed in mitogen stimulated T cells; GTP binding protein GEM; GTP binding protein overexpressed in skeletal muscle; Kinase inducible Ras like protein; KIR; MGC26294; RAS like protein KIR; GEM_HUMAN.

Human,Mouse,Rat,Dog,Pig,Cow,Horse

IHC,IF,ICC

IHC 1:100-500
IF 1:100-500
ICC 1:100-1:400

34kD

2669

P55040

https://www.uniprot.org/uniprotkb/P55040

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.

KLH conjugated synthetic peptide derived from human GEM

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.