Connexin 43 Rabbit pAb_经典一抗 -

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蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC0651

货号规格价格(￥)

PC0651S 50ul

PC0651M 100ul

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Connexin 43 Rabbit pAb

Datasheet

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq].

Alternative Names

Connexin 43; connexin43; Connexin43v; Cx 43v; CX43; CX 43; CX-43; DFNB38; Gap junction 43 kDa heart protein;Connexin-43; Gap junction alpha 1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; GJA1; GJA-1; GJAL; HGNC:4282; HGNC:8112; Oculodentodigital dysplasia; ODD; ODOD; SDTY3; Syndactyly type III; CXA1_HUMAN.

Reactivity

Human,Mouse,Rat,Chicken,Dog,Cow

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

42kD

Gene ID

2697

UniProt

P17302

Uniprot link

https://www.uniprot.org/uniprotkb/P17302

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.

Specificity

Expressed in the heart and fetal cochlea.

Immunogen

KLH conjugated synthetic peptide derived from human Connexin-43

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

IHC
IF
WB
ICC/IF
FC

Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Connexin 43) Polyclonal Antibody, Unconjugated (PC0651) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Connexin 43) Polyclonal Antibody, Unconjugated (PC0651) at 1:400 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.

Tissue/cell: rat heart tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer used at 1:200 dilution for 40 minutes at 37°C.

	Sample: Mcf-7 Cell Lysate at 40 ug Primary: Anti-Connexin(PC0651) at 1/300 dilution Secondary: Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 42 kD Observed band size: 43 kD
	Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Heart (Rat) Lysate at 40 ug Lane 3: Cerebrum (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Connexin 43 (PC0651) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 45 kD

	Tissue/cell: MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Connexin 43) polyclonal Antibody, Unconjugated (PC0651) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
	Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Connexin 43) polyclonal Antibody, Unconjugated (PC0651) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control (blue line):Hela(blue). Primary Antibody (green line): Rabbit Anti-Connexin 43 antibody(PC0651) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC. Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.