Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteosomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteosomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteosomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination.

ADMFD;
AIF4;
AIP4;
Atrophin 1 interacting protein 4;
Atrophin-1-interacting protein 4;
dJ468O1.1;
dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4));
dJ468O1.1 atrophin 1 interacting protein 4 AIP4;
E3 ubiquitin protein ligase Itchy homolog;
E3 ubiquitin-protein ligase Itchy homolog;
EC 6.3.2;
Itch;
ITCH_HUMAN;
Itchy E3 ubiquitin protein ligase;
Itchy E3 ubiquitin protein ligase homolog;
Itchy E3 ubiquitin protein ligase homolog mouse;
Itchy E3 ubiquitin protein ligase, mouse, homolog of;
Itchy homolog E3 ubiquitin protein ligase;
Itchy mouse homolog E3 ubiquitin protein ligase;
NAPP1;
NFE2 associated polypeptide 1;
NFE2-associated polypeptide 1;
Ubiquitin protein ligase ITCH.

Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep

WB,IHC,IF,ICC

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400

103kD

83737

Q96J02

https://www.uniprot.org/uniprotkb/Q96J02

Cell membrane. Cytoplasm. Nucleus. Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1.

Widely expressed.

KLH conjugated synthetic peptide derived from human ITCH/AIP4

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.