Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16.

Androgen receptor coactivator 55 kDa protein; Androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; Hic-5; Hydrogen peroxide-inducible clone 5 protein; TGFB1I1; TGFI1_HUMAN; Transforming growth factor beta 1 induced transcript 1; Transforming growth factor beta-1-induced transcript 1 protein; TSC 5.

Mouse,Human,Rat,Chicken,Dog,Cow,Horse,Rabbit,Sheep

IHC,IF,ICC

IHC 1:100-500
IF 1:100-500
ICC 1:100-1:400

50kD

7041

O43294

https://www.uniprot.org/uniprotkb/O43294

Cell junction; focal adhesion. Nucleus matrix. Cytoplasm; cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.

Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).

KLH conjugated synthetic peptide derived from human TGFB1I1/HIC5

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.