Alpha-synuclein (nitro-Tyr39) Rabbit pAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC9587

货号规格价格(￥)

PC9587S 50ul

PC9587M 100ul

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Alpha-synuclein (nitro-Tyr39) Rabbit pAb

Datasheet

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016].

Alternative Names

SYUA_HUMAN; nitrated Alpha-synuclein (nitrated-Tyr39); Alpha-synuclein 3-nitrotyrosines (Tyr39); Alpha-synuclein 3-nitrotyrosines Tyr39; Alpha synuclein 3-nitrotyrosines Tyr39; nitrated Alpha-synuclein (nitro-Tyr39); Alpha-synuclein (nitro-Tyr39); Alpha-synuclein (nitro Tyr39); SNCA; SYUA_HUMAN; Alpha synuclein; Alpha-synuclein, isoform NACP140; alpha SYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid , precursor of; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease (autosomal dominant, Lewy body) 4; Parkinson disease familial 1; Syn; Snca synuclein, alpha (non A4 component of amyloid precursor); Synuclein alpha; Synuclein, alpha (non A4 component of amyloid precursor); Synuclein-α; Synuclein α.

Reactivity

Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig

Application

IHC,IF,ICC,FC

Recommended Dilution

IHC 1:100-500
IF 1:100-500
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

15kD

Gene ID

20617

UniProt

Q16143

Uniprot link

https://www.uniprot.org/uniprotkb/Q16143

Subcellular location

Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Note=Membrane-bound in dopaminergic neurons.

Specificity

Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

Immunogen

KLH conjugated Synthesised nitrylpeptide derived from human SNCA around the nitrosation site of Tyr39

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-Alpha-synuclein (nitro-Tyr39) antibody (PC9587) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha-synuclein (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (PC9587) at 1:400 overnight at 4°C, followed by a conjugated secondary (A10026) for 20 minutes and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha-synuclein (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (PC9587) at 1:500 overnight at 4°C, followed by a conjugated secondary (A10026) for 20 minutes and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha-synuclein (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (PC9587) at 1:500 overnight at 4°C, followed by a conjugated secondary (A10026) for 20 minutes and DAB staining.