Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.

DNA topoisomerase 2 binding protein 1;
DNA topoisomerase 2-binding protein 1;
DNA topoisomerase II binding protein 1;
DNA topoisomerase II binding protein;
DNA topoisomerase II-beta-binding protein 1;
DNA topoisomerase II-binding protein 1;
DNA topoisomerase IIbeta binding protein 1;
Hypothetical protein KIAA0259 [Fragment];
KIAA0259;
TOP2BP1;
TOPB1_HUMAN;
TOPBP 1;
TopBP1;
Topoisomerase (DNA) II binding protein 1;
Topoisomerase II binding protein 1.

Mouse,Human,Rat,Dog,Cow,Rabbit,Sheep

WB

WB 1:500-2000

171kD

11073

Q92547

https://www.uniprot.org/uniprotkb/Q92547

Nucleus. Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation.

Highly expressed in heart, brain, placenta, lung and kidney.

KLH conjugated synthetic peptide derived from human TopBP1

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.