ZNF307/ZKSCAN3 Rabbit pAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC1940

货号规格价格(￥)

PC1940S 50ul

PC1940M 100ul

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-ZNF307/ZKSCAN3 Rabbit pAb

Datasheet

Background

ZNF307 contains 1 SCAN box domain, 1 KRAB domain and 7 C2H2-type zinc fingers. It belongs to the Krueppel C2H2-type zinc-finger protein family and may be involved in transcriptional regulation. [SUBCELLULAR LOCATION] Nucleus. Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.

Alternative Names

zinc finger with KRAB and SCAN domains 4; Zinc finger protein 307; P373c6.1; ZKSCAN4; FLJ32136; P1P373C6; p373c6.1 (novel C2H2 type zinc finger protein); p373c6.1; Zinc finger protein 307; Zinc finger with KRAB and SCAN domains 4; ZKSCAN4; ZKSC4_HUMAN;dJ874C20.1;
mszf35;
SCAN-KRAB-zinc finger protein;
Skz1;
Zf47;
Zfp-47;
ZFP306;
Zfp307;
Zfp47;
Zinc finger and SCAN domain containing protein 13;
Zinc finger and SCAN domain-containing protein 13;
Zinc finger protein 306;
Zinc finger protein 306, isoform CRA_a;
Zinc finger protein 307;
Zinc finger protein 309;
Zinc finger protein 47 homolog;
Zinc finger protein with KRAB and SCAN domains 3;
Zinc finger protein with KRAB and SCAN domains 3;
Zinc finger protein zfp47;
Zinc finger with KRAB and SCAN domains 3;
ZKSC3_HUMAN;
ZKSCAN3;
ZNF309;
ZSCAN13;
ZSCAN35.

Reactivity

Human,Mouse,Rat,Dog,Cow,Horse,Rabbit

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

62kD

Gene ID

387032

UniProt

Q969J2

Uniprot link

https://www.uniprot.org/uniprotkb/Q969J2

Subcellular location

Nucleus.

Specificity

Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.

Immunogen

KLH conjugated synthetic peptide derived from human ZNF307

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

IHC
WB
ICC/IF
FC

	Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(PC1940) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(PC1940) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(PC1940) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(PC1940) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining

Sample: K562(Human) Cell Lysate at 40 ug Primary: Anti-ZNF307/ZKSCAN3 (PC1940) at 1/300 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ZNF307/ZKSCAN3) polyclonal Antibody, Unconjugated (PC1940) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control（black line）:SH-SY5Y. Primary Antibody (green line): Rabbit Anti-ZNF307/ZKSCAN3 antibody (PC1940) Dilution:2ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.