SPARC Rabbit pAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC1133

货号规格价格(￥)

PC1133S 50ul

PC1133M 100ul

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-SPARC Rabbit pAb

Datasheet

Background

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011].

Alternative Names

AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin); Secreted protein acidic cysteine rich; SPRC; SPRC_HUMAN.

Reactivity

Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

33kD

Gene ID

6678

UniProt

P09486

Uniprot link

https://www.uniprot.org/uniprotkb/P09486

Subcellular location

Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.

Specificity

Immunogen

KLH conjugated synthetic peptide derived from human SPARC

Source

Rabbit

Clonality

Polyclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:400 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:400 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:400 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SPARC Polyclonal Antibody, Unconjugated(PC1133) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Formalin-fixed and paraffin embedded human ovarian tissue labeled with Anti-SPARC Polyclonal Antibody, Unconjugated (PC1133) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
	Tissue/cell: Human Meningioma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SPARC Polyclonal Antibody, Unconjugated(PC1133) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(A10026) and DAB staining
	Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (PC1133) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.

	Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-SPARC (PC1133) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD
	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-SPARC (PC1133) at 1/300 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD

	Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei
	Tissue/cell: mouse kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei

Blank control:U87MG. Primary Antibody (green line): Rabbit Anti-SPARC antibody (PC1133) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.