Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.

HEF1 (phospho S369); p-HEF1 (phospho S369); Cas like docking; Cas scaffolding protein family member 2;
CAS-L;
CAS2;
CasL;
CASL_HUMAN;
CASS2;
Crk associated substrate related;
Crk associated substrate related protein;
CRK-associated substrate-related protein;
dJ49G10.2 (Enhancer of Filamentation 1 (HEF1));
dJ49G10.2;
dJ761I2.1 (enhancer of filamentation (HEF1));
dJ761I2.1;
Enhancer of filamentation 1;
Enhancer of filamentation 1 p55;
HEF 1;
HEF1

Human,Cow,Horse,Sheep

WB

WB 1:500-2000

93kD

4739

Q14511

https://www.uniprot.org/uniprotkb/Q14511

Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
Enhancer of filamentation 1 p55: Cytoplasm, cytoskeleton, spindle.

Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.

KLH conjugated synthesised phosphopeptide derived from human HEF1 around the phosphorylation site of Ser369

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.