PCNA Rabbit mAb, Nuclear Loading Control

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

TC52347

货号规格价格(￥)

TC52347S 50ul

TC52347M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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标签/内参抗体-PCNA Rabbit mAb, Nuclear Loading Control

Datasheet

Background

Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

Alternative Names

Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen; PCNA_HUMAN.

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:1000-10000
IHC 1:200-1000
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

29kD

Gene ID

5111

UniProt

P12004

Uniprot link

https://www.uniprot.org/uniprotkb/P12004

Subcellular location

Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Specificity

Immunogen

Recombinant human PCNA protein

Source

Rabbit

Clonality

Recombinant

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(TC52347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.