Calreticulin Mouse mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MC51748

货号规格价格(￥)

MC51748S 50ul

MC51748M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Calreticulin Mouse mAb

Datasheet

Background

Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]

Alternative Names

Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400

Application Image

Mol Weight

44kD

Gene ID

811

UniProt

P27797

Uniprot link

https://www.uniprot.org/uniprotkb/P27797

Subcellular location

Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.

Specificity

Immunogen

Recombinant human CALR.

Source

Mouse

Clonality

Monoclonal

Isotype

IgG1,Κ

Purification

affinity purified by Protein G

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Sample: Lane 1: HepG2 cell lysates Lane 2: Jurkat cell lysates Lane 3: Hela cell lysates Lane 4: MCF-7 cell lysates Lane 5: SH-SY5Y cell lysates Lane 6: K562 cell lysates Lane 7: NIH/3T3 cell lysates Lane 8: C6 cell lysates Lane 9: CHO cell lysates Primary: Anti-Calreticulin (MC51748) at 1/2000 dilution Secondary: Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 56 kD
	Sample: Lane 1: Human SH-SY5Y cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human K562 cell lysates Lane 4: Human HL-60 cell lysates Lane 5: Human A431 cell lysates Lane 6: Human Jurkat cell lysates Lane 7: Human HepG2 cell lysates Lane 8: Human MCF-7 cell lysates Primary: Anti-Calreticulin (MC51748) at 1/1000 dilution Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific Predicted band size: 44 kDa Observed band size: 50 kDa
	Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Testis tissue lysates Primary: Anti-Calreticulin (MC51748) at 1/1000 dilution Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific Predicted band size: 44 kDa Observed band size: 55 kDa

	Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (MC51748) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.