Synaptophysin Rabbit mAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

TC52379

货号规格价格(￥)

TC52379S 50ul

TC52379M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Synaptophysin Rabbit mAb

Datasheet

Background

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Alternative Names

Major synaptic vesicle protein P38;
MRXSYP;
Syn p38;
SYP;
SYPH;
SYPH_HUMAN;
SypI.

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Application Image

Mol Weight

34kD

Gene ID

6855

UniProt

P08247

Uniprot link

https://www.uniprot.org/uniprotkb/P08247

Subcellular location

Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.

Specificity

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Immunogen

Recombinant human SYP protein

Source

Rabbit

Clonality

Recombinant

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

ICC/IF
IHC
WB

Blank control:N2A. Primary Antibody (green line): Rabbit Anti-Synaptophysin antibody (TC52379) Dilution: 1:50; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1:1000. Protocol The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

PC12 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

	Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:200 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synaptophysin) Monoclonal Antibody, Unconjugated (TC52379) at 1:100 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Rabbit）Cat#A10026instructions and DAB staining.

Sample: Lane 1: Mouse Cerebrum Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Rat Cerebrum Lysates Lane 4: Rat Cerebellum Lysates Primary: Anti-Synaptophysin (TC52379) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa