Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior (1). In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively (1). These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway (1). One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals (2,3). Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion (1-3). Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross (1). Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout (2-4). Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray (5,6).

Axon guidance receptor; Deleted in U twenty twenty; DUTT 1; DUTT1; FLJ21882; H Robo 1; H-Robo-1; hRobo 1; Robo 1; Robo1; ROBO1_HUMAN; Roundabout 1; Roundabout axon guidance receptor homolog 1; Roundabout homolog 1; Roundabout homolog1 precurser; Roundabout1; SAX 3; SAX3.

Rat,Human,Mouse

IHC,IF,ICC

IHC 1:50-1:200
IF 1:100-500
ICC 1:100-1:400

181kD

6091

Q9Y6N7

https://www.uniprot.org/uniprotkb/Q9Y6N7

Membrane.

Widely expressed, with exception of kidney.

KLH conjugated synthetic peptide derived from human Robo

Rabbit

Polyclonal

IgG

affinity purified by Protein A

Liquid

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.