SPHK2 Mouse mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MC33868

货号规格价格(￥)

MC33868S 50ul

MC33868M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-SPHK2 Mouse mAb

Datasheet

Background

This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Alternative Names

sphingosine kinase 2; SK 2; SPK 2; C76851; MGC102297; SPHK2_HUMAN.

Reactivity

Human,Mouse,Rat

Application

IHC,IF,ICC

Recommended Dilution

IHC 1:400-800
IF 1:100-500
ICC 1:100-1:400

Application Image

Mol Weight

72kD

Gene ID

56848

UniProt

Q9NRA0

Uniprot link

https://www.uniprot.org/uniprotkb/Q9NRA0

Subcellular location

Isoform 1: Cytoplasm. Membrane. Isoform 2: Lysosome membrane.

Specificity

Immunogen

KLH conjugated synthetic peptide derived from human SPHK2

Source

Mouse

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (MC33868) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Monoclonal Antibody, Unconjugated (ascites of MC33868) at 1:2000 overnight at 4°C, followed by operating according to SignalEnhance IHC KIT（Mouse）Cat#A10027 instructions and DAB staining.