FASN Mouse mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MC51155

货号规格价格(￥)

MC51155S 50ul

MC51155M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-FASN Mouse mAb

Datasheet

Background

FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation.

Alternative Names

fatty acid synthase; FASN; FAS; FA Synthase 1; MGC14367; MGC15706; OA-519; Fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; Oleoyl-[acyl-carrier-protein] hydrolase; FAS_MHUMAN.

Reactivity

Human,Mouse

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:500-5000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400

Application Image

Mol Weight

274kD

Gene ID

2194

UniProt

P49327

Uniprot link

https://www.uniprot.org/uniprotkb/P49327

Subcellular location

Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Specificity

Ubiquitous. Prominent expression in brain, lung, and liver.

Immunogen

KLH conjugated synthetic peptide derived from human FASN

Source

Mouse

Clonality

Monoclonal

Isotype

IgM

Purification

affinity purified by Protein AGL

Formulation

Liquid

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentrate

Storage Condition

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (MC51155) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (MC51155) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (MC51155) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (MC51155) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(A10027) instructions and DAB staining.

Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Primary: Anti-FASN (MC51155) at 1/1000 dilution Secondary: Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 274 kDa Observed band size: 270 kDa