Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore.

HsPK21; NEK2; NEK2A; NLK1.

Human

WB

WB 1:1000-1:2000

52kD

4751

P51955

https://www.uniprot.org/uniprotkb/P51955

Isoform 1: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere (By similarity). Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.
Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic. <> Isoform 4: Nucleus. Cytoplasm, cytoskeleton, centrosome. Note=Predominantly brnuclear.

Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.

KLH conjugated synthetic peptide derived from human NEK2

Rabbit

Recombinant

IgG

affinity purified by Protein A

Liquid

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.