TC62916

货号 规格 价格(¥)
TC62916S 50ul
TC62916M 100ul

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

ABMART实验方案下载

   蛋白质免疫共沉淀

   蛋白质印迹

   免疫荧光

   免疫组化

   膜再生

   植物BPP法变性蛋白提取

   组织制备成单细胞悬液

经典一抗-Phospho-Nucleolin (Thr84) Rabbit mAb

Datasheet

Background

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Alternative Names

NCL; Nucl; Nucleolin; Protein C23.

Reactivity

Human

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:1000-1:2000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:100-1:400

Application Image

Mol Weight

77kD

Gene ID

4691

UniProt

P19338

Uniprot link

Subcellular location

Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Specificity

Immunogen

KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T84

Source

Rabbit

Clonality

Recombinant

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Buffer

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Concentrate

Storage Condition

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

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北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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