E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, leading to degradation of nascent peptide chains . ZNF598 is activated when ribosomes are stalled within an mRNA following translation of prematurely polyadenylated mRNAs . Acts as a ribosome collision sensor: specifically recognizes and binds collided di-ribosome, which arises when a trailing ribosome encounters a slower leading ribosome, leading to terminally arrest translation . Following binding to colliding ribosomes, mediates monoubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS3/uS3, and ''Lys-63''-linked polyubiquitination of RPS20/uS10 . Polyubiquitination of RPS20/uS10 promotes recruitment of the RQT (ribosome quality control trigger) complex, which drives the disassembly of stalled ribosomes, followed by degradation of nascent peptides . E3 ubiquitin-protein ligase activity is dependent on the E2 ubiquitin-conjugating enzyme UBE2D3 . Also acts as an adapter that recruits the 4EHP-GYF2 complex to mRNAs . Independently of its role in RQC, may also act as a negative regulator of interferon-stimulated gene (ISG) expression . .; (Microbial infection) Required for poxvirus protein synthesis by mediating ubiquitination of RPS10/eS10 and RPS20/uS10 . Poxvirus encoding mRNAs contain unusual 5'' poly(A) leaders and ZNF598 is required for their translational efficiency, possibly via its ability to suppress readthrough or sliding on shorter poly(A) tracts . .

E3 ubiquitin-protein ligase ZNF598 (EC 2.3.2.27) (Zinc finger protein 598),ZNF598

q86uk7

Mouse

Monoclonal

IgG

Human

WB

WB 1:2000

99kD

Supplied as solution in phosphate buffered saline containing 0.09% sodium azide

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.