GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

65 kDa glutamic acid decarboxylase; 67 kDa glutamic acid decarboxylase; EC 4.1.1.15; FLJ45882; GAD 65; GAD 67; GAD-65; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 1; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65

q99259

Human,Mouse,Rat

WB,IF,ICC

WB 1:500-1000
IF 1:100-500
ICC 1:100-300

65kD

Goat

Recombinant Human GAD65/GAD67

Ammonium sulfate precipitation&Antigen affinity chromatography

Liquid

PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA

Store at -20℃. Stable for one year

~1mg/ml