E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the ''Lys-63''-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing ''Lys-48''-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the ''Lys-63''-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes ''Lys-63''-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Promotes the formation of ''Lys-63''-linked polyubiquitin chains via interactions with the specific ubiquitin-conjugating UBE2N/UBC13 and ubiquitinates non-histone substrates such as PCNA. Substrates that are polyubiquitinated at ''Lys-63'' are usually not targeted for degradation. Also catalyzes the formation of ''Lys-48''-linked polyubiquitin chains via interaction with the ubiquitin-conjugating UBE2L6/UBCH8, leading to degradation of substrate proteins such as CHEK2, JMJD2A/KDM4A and KU80/XRCC5: it is still unclear how the preference toward ''Lys-48''- versus ''Lys-63''-linked ubiquitination is regulated but it could be due to RNF8 ability to interact with specific E2 specific ligases.

E3 ubiquitin-protein ligase RNF8
hRNF8
RING finger protein 8
RING-type E3 ubiquitin transferase RNF8
RNF8
KIAA0646

o76064

WB,IHC

WB 1:2000
IHC 1:500

Human

Predicted size: 55 kDa

Rabbit

Chromosome, Cytoplasm, Nucleus, Telomere.

Protein A affinity purified.

Recombinant Rabbit monoclonal

IgG

Liquid

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.