Polynucleotide kinase that can phosphorylate the 5''-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3''-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5''-terminus of the tRNA 3''-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3''-end formation. Also phosphorylates the 5''-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5''-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Polyribonucleotide 5''-hydroxyl-kinase Clp1
Polyadenylation factor Clp1
Polynucleotide kinase Clp1
Pre-mRNA cleavage complex II protein Clp1
CLP1
HEAB

q92989

WB,IHC,FC

WB 1:1000
IHC 1:200
FC 1:1000

Human,Mouse,Rat

Predicted size: 48 kDa

Rabbit

Nucleus.

Protein A affinity purified.

Recombinant Rabbit monoclonal

IgG

Liquid

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.