15 μg of HCT116 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. ME084489 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. TALDO1 band was visualized using ECL Western Blotting Substrate.
Result: ME084489 can detect TALDO1 by Western blotting.

IHC-P analysis of colon tissue by TALDO1 antibody.
IHC-P was performed using sections of the formalin-fixed paraffin-embedded colon tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with TALDO1 antibody at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells and cells in lamina propria are positively stained at the nuclei.