NAD-dependent protein-lysine deacylase that can act both as a deacetylase or deacylase , depending on the context . Specifically mediates deacetylation of histone H3 at 'Lys-18' . In contrast to other histone deacetylases, displays strong preference for a specific histone mark, H3K18Ac, directly linked to control of gene expression . H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors; SIRT7 thereby acts as a transcription repressor . Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells . Also able to mediate deacetylation of histone H3 at 'Lys-36' in the context of nucleosomes . Also mediates deacetylation of non-histone proteins, such as ATM, CDK9, DDX21, DDB1, FBL, FKBP5/FKBP51, GABPB1, RAN, RRP9/U3-55K and POLR1E/PAF53 . Enriched in nucleolus where it stimulates transcription activity of the RNA polymerase I complex . Acts by mediating the deacetylation of the RNA polymerase I subunit POLR1E/PAF53, thereby promoting the association of RNA polymerase I with the rDNA promoter region and coding region . In response to metabolic stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased RNA polymerase I transcription . Required to restore the transcription of ribosomal RNA at the exit from mitosis . Promotes pre-ribosomal RNA cleavage at the 5'-terminal processing site by mediating deacetylation of RRP9/U3-55K, a core subunit of the U3 snoRNP complex . Mediates 'Lys-37' deacetylation of Ran, thereby regulating the nuclear export of NF-kappa-B subunit RELA/p65 . Acts as a regulator of DNA damage repair by mediating deacetylation of ATM during the late stages of DNA damage response, promoting ATM dephosphorylation and deactivation . Suppresses the activity of the DCX E3 ubiquitin-protein ligase complexes by mediating deacetylation of DDB1, which prevents the interaction between DDB1 and CUL4 . Activates RNA polymerase II transcription by mediating deacetylation of CDK9, thereby promoting 'Ser-2' phosphorylation of the C-terminal domain of RNA polymerase II . Deacetylates FBL, promoting histone-glutamine methyltransferase activity of FBL . Acts as a regulator of mitochondrial function by catalyzing deacetylation of GABPB1 . Regulates Akt/AKT1 activity by mediating deacetylation of FKBP5/FKBP51 . Required to prevent R-loop-associated DNA damage and transcription-associated genomic instability by mediating deacetylation and subsequent activation of DDX21, thereby overcoming R-loop-mediated stalling of RNA polymerases . In addition to protein deacetylase activity, also acts as a protein-lysine deacylase . Acts as a protein depropionylase by mediating depropionylation of Osterix , thereby regulating bone formation by osteoblasts . Acts as a histone deglutarylase by mediating deglutarylation of histone H4 on 'Lys-91' ; a mark that destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes . Acts as a histone desuccinylase: in response to DNA damage, recruited to DNA double-strand breaks and catalyzes desuccinylation of histone H3 on 'Lys-122' , thereby promoting chromatin condensation and DSB repair . Also promotes DSB repair by promoting H3K18Ac deacetylation, regulating non-homologous end joining . Along with its role in DNA repair, required for chromosome synapsis during prophase I of female meiosis by catalyzing H3K18Ac deacetylation . Involved in transcriptional repression of LINE-1 retrotransposon via H3K18Ac deacetylation, and promotes their association with the nuclear lamina . Required to stabilize ribosomal DNA heterochromatin and prevent cellular senescence induced by rDNA instability . Acts as a negative regulator of SIRT1 by preventing autodeacetylation of SIRT1, restricting SIRT1 deacetylase activity .

NAD-dependent protein deacetylase sirtuin-7 (EC 2.3.1.286) (NAD-dependent protein deacylase sirtuin-7) (EC 2.3.1.-) (Regulatory protein SIR2 homolog 7) (SIR2-like protein 7)

q9nrc8, q8bkj9, b2rz55

Human, Mouse, Rat

WB,IHC,IP

WB 1:1000
IHC 1:100
IP 1:20-1:50

~45 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.