Substrate-specific adapter of a BCR E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination . KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes . In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes . In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR complex and activation of NFE2L2/NRF2 . The BCR complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation . The BCR complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome .

Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19)

q14145

Human,Mouse

WB,IHC

WB 1:1000-1:15000
IHC 1:200-1:500

~70 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.