Component of the cleavage factor Im complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs . CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals . Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation producing mRNAs with variable 3'-end formation . The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region for a huge number of pre-mRNAs . CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery . Plays a role in mRNA export . ; Binds HIV-1 capsid-nucleocapsid complexes and might thereby promote the integration of the virus in the nucleus of dividing cells .

Cleavage and polyadenylation specificity factor subunit 6 (Cleavage and polyadenylation specificity factor 68 kDa subunit) (CPSF 68 kDa subunit) (Cleavage factor Im complex 68 kDa subunit) (CFIm68) (Pre-mRNA cleavage factor Im 68 kDa subunit) (Protein HPBRII-4/7)

q16630

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:2000-1:20000
IHC 1:100-1:200
IF 1:100-1:200
ICC 1:100-1:200
IP 1:10-1:100
FC 1:10-1:100

~60 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.