Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing . Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo-nucleotide triphosphates . Unwinds numerous nucleic acid substrates such as double-stranded DNA and RNA, DNA:RNA hybrids, DNA and RNA forks composed of either partially complementary DNA duplexes or DNA:RNA hybrids, respectively, and also DNA and RNA displacement loops , triplex-helical DNA structure and DNA and RNA-based G-quadruplexes . Binds dsDNA, single-stranded DNA , dsRNA, ssRNA and poly-containing RNA . Also binds to circular dsDNA or dsRNA of either linear and/or circular forms and stimulates the relaxation of supercoiled DNAs catalyzed by topoisomerase TOP2A . Plays a role in DNA replication at origins of replication and cell cycle progression . Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1 . Binds to the CDKN2A promoter . Plays several roles in post-transcriptional regulation of gene expression . In cooperation with NUP98, promotes pre-mRNA alternative splicing activities of a subset of genes . As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms . Also acts as a nuclear resolvase that is able to bind and neutralize harmful massive secondary double-stranded RNA structures formed by inverted-repeat Alu retrotransposon elements that are inserted and transcribed as parts of genes during the process of gene transposition . Involved in the positive regulation of nuclear export of constitutive transport element -containing unspliced mRNA . Component of the coding region determinant -mediated complex that promotes cytoplasmic MYC mRNA stability . Plays a role in mRNA translation . Positively regulates translation of selected mRNAs through its binding to post-transcriptional control element in the 5'-untranslated region . Involved with LARP6 in the translation stimulation of type I collagen mRNAs for CO1A1 and CO1A2 through binding of a specific stem-loop structure in their 5'-UTRs . Stimulates LIN28A-dependent mRNA translation probably by facilitating ribonucleoprotein remodeling during the process of translation . Plays also a role as a small interfering -loading factor involved in the RNA-induced silencing complex loading complex assembly, and hence functions in the RISC-mediated gene silencing process . Binds preferentially to short double-stranded RNA, such as those produced during rotavirus intestinal infection . This interaction may mediate NLRP9 inflammasome activation and trigger inflammatory response, including IL18 release and pyroptosis . Finally, mediates the attachment of heterogeneous nuclear ribonucleoproteins to actin filaments in the nucleus . ; Plays a role in HIV-1 replication and virion infectivity . Enhances HIV-1 transcription by facilitating the binding of RNA polymerase II holoenzyme to the proviral DNA . Binds to the HIV-1 TAR RNA and stimulates HIV-1 transcription of transactivation response element -containing mRNAs . Involved also in HIV-1 mRNA splicing and transport . Positively regulates HIV-1 gag mRNA translation, through its binding to post-transcriptional control element in the 5'-untranslated region . Binds to a HIV-1 double-stranded RNA region of the primer binding site -segment of the 5'-UTR, and hence stimulates DHX9 incorporation into virions and virion infectivity . Also plays a role as a cytosolic viral MyD88-dependent DNA and RNA sensors in plasmacytoid dendritic cells , and hence induce antiviral innate immune responses . Binds to viral single-stranded DNA unmethylated C-phosphate-G oligonucleotide .

ATP-dependent RNA helicase A (EC 3.6.4.13) (DEAH box protein 9) (DExH-box helicase 9) (Leukophysin) (LKP) (Nuclear DNA helicase II) (NDH II) (RNA helicase A)

q08211

Human,Mouse,Rat

WB,IHC,IF,ICC,FC

WB 1:2000-1:20000
IHC 1:100-1:200
IF 1:50
ICC 1:50
FC 1:20

~141 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.