Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts to release precursor miRNA in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs . The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding . Specifically recognizes and binds N6-methyladenosine -containing pri-miRNAs, a modification required for pri-miRNAs processing . Involved in the silencing of embryonic stem cell self-renewal . Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks .

Microprocessor complex subunit DGCR8 (DiGeorge syndrome critical region 8)

q8wyq5

Human,Mouse,Rat

WB,IF,ICC,IP,FC

WB 1:1000
IF 1:500-1:1000
ICC 1:500-1:1000
IP 1:50
FC 1:200-1:1000

~87 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.