Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase . Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring . May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression . Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L . Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation . May be involved in regulation of DNA replication by acting as scaffold for MCM2 . Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation . May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells . May act as a carrier protein of GJA1 for its transport to the nucleus . May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA chromatin, preferentially with rRNA promoter and transcribed regions . Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 .

A-kinase anchor protein 8 (AKAP-8) (A-kinase anchor protein 95 kDa) (AKAP 95)

o43823

Human,Mouse,Rat

WB,IHC,IF,ICC

WB 1:1000-1:5000
IHC 1:100-1:200
IF 1:100-1:200
ICC 1:100-1:200

~77 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.